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<t>Curcumin</t> rescues <t>Erastin</t> induced ferroptosis and restores decidualization in telomerase‐immortalized human endometrial stromal cells (T hESCs). (A) Representative transmission electron microscopy (TEM) images of mitochondrial ultrastructure in T hESCs under indicated treatments indicated shrunken mitochondria with condensed membranes, characteristic of ferroptosis. (B–D) Biochemical assessment of ferroptosis markers: Malondialdehyde (MDA) content (B), superoxide dismutase (SOD) activity (C), and the reduced/oxidized glutathione (GSH/GSSG) ratio (D) ( n = 6). (E–H) Representative immunofluorescence images showing intracellular ferrous iron (Fe 2+ ) (E), and expression of TFRC (F), ACSL4 (G), and SLC7A11 (H) (scale bar: 50 μm). (I–L) Quantification of fluorescence intensity for Fe 2+ (I), TFRC (J), ACSL4 (K), and SLC7A11 (L) ( n = 3). (M, N) ELISA was used to quantify the level of IGFBP1 (M) and PRL (N) from each group ( n = 6). Data are presented as means ± SEM. Statistical significance is determined by one‐way ANOVA (Dunnett's post‐test). ** p < 0.01 (normal control vs. model group); # p < 0.05, ## p < 0.01 (treatment groups vs. model group).
Curcumin N0005, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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curcumin  (MedChemExpress)
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<t>Curcumin</t> rescues <t>Erastin</t> induced ferroptosis and restores decidualization in telomerase‐immortalized human endometrial stromal cells (T hESCs). (A) Representative transmission electron microscopy (TEM) images of mitochondrial ultrastructure in T hESCs under indicated treatments indicated shrunken mitochondria with condensed membranes, characteristic of ferroptosis. (B–D) Biochemical assessment of ferroptosis markers: Malondialdehyde (MDA) content (B), superoxide dismutase (SOD) activity (C), and the reduced/oxidized glutathione (GSH/GSSG) ratio (D) ( n = 6). (E–H) Representative immunofluorescence images showing intracellular ferrous iron (Fe 2+ ) (E), and expression of TFRC (F), ACSL4 (G), and SLC7A11 (H) (scale bar: 50 μm). (I–L) Quantification of fluorescence intensity for Fe 2+ (I), TFRC (J), ACSL4 (K), and SLC7A11 (L) ( n = 3). (M, N) ELISA was used to quantify the level of IGFBP1 (M) and PRL (N) from each group ( n = 6). Data are presented as means ± SEM. Statistical significance is determined by one‐way ANOVA (Dunnett's post‐test). ** p < 0.01 (normal control vs. model group); # p < 0.05, ## p < 0.01 (treatment groups vs. model group).
Curcumin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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<t>Curcumin</t> rescues <t>Erastin</t> induced ferroptosis and restores decidualization in telomerase‐immortalized human endometrial stromal cells (T hESCs). (A) Representative transmission electron microscopy (TEM) images of mitochondrial ultrastructure in T hESCs under indicated treatments indicated shrunken mitochondria with condensed membranes, characteristic of ferroptosis. (B–D) Biochemical assessment of ferroptosis markers: Malondialdehyde (MDA) content (B), superoxide dismutase (SOD) activity (C), and the reduced/oxidized glutathione (GSH/GSSG) ratio (D) ( n = 6). (E–H) Representative immunofluorescence images showing intracellular ferrous iron (Fe 2+ ) (E), and expression of TFRC (F), ACSL4 (G), and SLC7A11 (H) (scale bar: 50 μm). (I–L) Quantification of fluorescence intensity for Fe 2+ (I), TFRC (J), ACSL4 (K), and SLC7A11 (L) ( n = 3). (M, N) ELISA was used to quantify the level of IGFBP1 (M) and PRL (N) from each group ( n = 6). Data are presented as means ± SEM. Statistical significance is determined by one‐way ANOVA (Dunnett's post‐test). ** p < 0.01 (normal control vs. model group); # p < 0.05, ## p < 0.01 (treatment groups vs. model group).
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<t>Curcumin</t> rescues <t>Erastin</t> induced ferroptosis and restores decidualization in telomerase‐immortalized human endometrial stromal cells (T hESCs). (A) Representative transmission electron microscopy (TEM) images of mitochondrial ultrastructure in T hESCs under indicated treatments indicated shrunken mitochondria with condensed membranes, characteristic of ferroptosis. (B–D) Biochemical assessment of ferroptosis markers: Malondialdehyde (MDA) content (B), superoxide dismutase (SOD) activity (C), and the reduced/oxidized glutathione (GSH/GSSG) ratio (D) ( n = 6). (E–H) Representative immunofluorescence images showing intracellular ferrous iron (Fe 2+ ) (E), and expression of TFRC (F), ACSL4 (G), and SLC7A11 (H) (scale bar: 50 μm). (I–L) Quantification of fluorescence intensity for Fe 2+ (I), TFRC (J), ACSL4 (K), and SLC7A11 (L) ( n = 3). (M, N) ELISA was used to quantify the level of IGFBP1 (M) and PRL (N) from each group ( n = 6). Data are presented as means ± SEM. Statistical significance is determined by one‐way ANOVA (Dunnett's post‐test). ** p < 0.01 (normal control vs. model group); # p < 0.05, ## p < 0.01 (treatment groups vs. model group).
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<t>Curcumin</t> rescues <t>Erastin</t> induced ferroptosis and restores decidualization in telomerase‐immortalized human endometrial stromal cells (T hESCs). (A) Representative transmission electron microscopy (TEM) images of mitochondrial ultrastructure in T hESCs under indicated treatments indicated shrunken mitochondria with condensed membranes, characteristic of ferroptosis. (B–D) Biochemical assessment of ferroptosis markers: Malondialdehyde (MDA) content (B), superoxide dismutase (SOD) activity (C), and the reduced/oxidized glutathione (GSH/GSSG) ratio (D) ( n = 6). (E–H) Representative immunofluorescence images showing intracellular ferrous iron (Fe 2+ ) (E), and expression of TFRC (F), ACSL4 (G), and SLC7A11 (H) (scale bar: 50 μm). (I–L) Quantification of fluorescence intensity for Fe 2+ (I), TFRC (J), ACSL4 (K), and SLC7A11 (L) ( n = 3). (M, N) ELISA was used to quantify the level of IGFBP1 (M) and PRL (N) from each group ( n = 6). Data are presented as means ± SEM. Statistical significance is determined by one‐way ANOVA (Dunnett's post‐test). ** p < 0.01 (normal control vs. model group); # p < 0.05, ## p < 0.01 (treatment groups vs. model group).
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<t>Curcumin</t> rescues <t>Erastin</t> induced ferroptosis and restores decidualization in telomerase‐immortalized human endometrial stromal cells (T hESCs). (A) Representative transmission electron microscopy (TEM) images of mitochondrial ultrastructure in T hESCs under indicated treatments indicated shrunken mitochondria with condensed membranes, characteristic of ferroptosis. (B–D) Biochemical assessment of ferroptosis markers: Malondialdehyde (MDA) content (B), superoxide dismutase (SOD) activity (C), and the reduced/oxidized glutathione (GSH/GSSG) ratio (D) ( n = 6). (E–H) Representative immunofluorescence images showing intracellular ferrous iron (Fe 2+ ) (E), and expression of TFRC (F), ACSL4 (G), and SLC7A11 (H) (scale bar: 50 μm). (I–L) Quantification of fluorescence intensity for Fe 2+ (I), TFRC (J), ACSL4 (K), and SLC7A11 (L) ( n = 3). (M, N) ELISA was used to quantify the level of IGFBP1 (M) and PRL (N) from each group ( n = 6). Data are presented as means ± SEM. Statistical significance is determined by one‐way ANOVA (Dunnett's post‐test). ** p < 0.01 (normal control vs. model group); # p < 0.05, ## p < 0.01 (treatment groups vs. model group).
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curcumin  (Loba Chemie)
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<t>Curcumin</t> rescues <t>Erastin</t> induced ferroptosis and restores decidualization in telomerase‐immortalized human endometrial stromal cells (T hESCs). (A) Representative transmission electron microscopy (TEM) images of mitochondrial ultrastructure in T hESCs under indicated treatments indicated shrunken mitochondria with condensed membranes, characteristic of ferroptosis. (B–D) Biochemical assessment of ferroptosis markers: Malondialdehyde (MDA) content (B), superoxide dismutase (SOD) activity (C), and the reduced/oxidized glutathione (GSH/GSSG) ratio (D) ( n = 6). (E–H) Representative immunofluorescence images showing intracellular ferrous iron (Fe 2+ ) (E), and expression of TFRC (F), ACSL4 (G), and SLC7A11 (H) (scale bar: 50 μm). (I–L) Quantification of fluorescence intensity for Fe 2+ (I), TFRC (J), ACSL4 (K), and SLC7A11 (L) ( n = 3). (M, N) ELISA was used to quantify the level of IGFBP1 (M) and PRL (N) from each group ( n = 6). Data are presented as means ± SEM. Statistical significance is determined by one‐way ANOVA (Dunnett's post‐test). ** p < 0.01 (normal control vs. model group); # p < 0.05, ## p < 0.01 (treatment groups vs. model group).
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curcumin  (Tokyo Chemical Industry)
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Tokyo Chemical Industry curcumin
<t>Curcumin</t> rescues <t>Erastin</t> induced ferroptosis and restores decidualization in telomerase‐immortalized human endometrial stromal cells (T hESCs). (A) Representative transmission electron microscopy (TEM) images of mitochondrial ultrastructure in T hESCs under indicated treatments indicated shrunken mitochondria with condensed membranes, characteristic of ferroptosis. (B–D) Biochemical assessment of ferroptosis markers: Malondialdehyde (MDA) content (B), superoxide dismutase (SOD) activity (C), and the reduced/oxidized glutathione (GSH/GSSG) ratio (D) ( n = 6). (E–H) Representative immunofluorescence images showing intracellular ferrous iron (Fe 2+ ) (E), and expression of TFRC (F), ACSL4 (G), and SLC7A11 (H) (scale bar: 50 μm). (I–L) Quantification of fluorescence intensity for Fe 2+ (I), TFRC (J), ACSL4 (K), and SLC7A11 (L) ( n = 3). (M, N) ELISA was used to quantify the level of IGFBP1 (M) and PRL (N) from each group ( n = 6). Data are presented as means ± SEM. Statistical significance is determined by one‐way ANOVA (Dunnett's post‐test). ** p < 0.01 (normal control vs. model group); # p < 0.05, ## p < 0.01 (treatment groups vs. model group).
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Curcumin rescues Erastin induced ferroptosis and restores decidualization in telomerase‐immortalized human endometrial stromal cells (T hESCs). (A) Representative transmission electron microscopy (TEM) images of mitochondrial ultrastructure in T hESCs under indicated treatments indicated shrunken mitochondria with condensed membranes, characteristic of ferroptosis. (B–D) Biochemical assessment of ferroptosis markers: Malondialdehyde (MDA) content (B), superoxide dismutase (SOD) activity (C), and the reduced/oxidized glutathione (GSH/GSSG) ratio (D) ( n = 6). (E–H) Representative immunofluorescence images showing intracellular ferrous iron (Fe 2+ ) (E), and expression of TFRC (F), ACSL4 (G), and SLC7A11 (H) (scale bar: 50 μm). (I–L) Quantification of fluorescence intensity for Fe 2+ (I), TFRC (J), ACSL4 (K), and SLC7A11 (L) ( n = 3). (M, N) ELISA was used to quantify the level of IGFBP1 (M) and PRL (N) from each group ( n = 6). Data are presented as means ± SEM. Statistical significance is determined by one‐way ANOVA (Dunnett's post‐test). ** p < 0.01 (normal control vs. model group); # p < 0.05, ## p < 0.01 (treatment groups vs. model group).

Journal: Food Science & Nutrition

Article Title: Curcumin Attenuates Decidual Stromal Cell Ferroptosis and Restores Impaired Decidualization in Recurrent Spontaneous Abortion by Targeting BRD4

doi: 10.1002/fsn3.72205

Figure Lengend Snippet: Curcumin rescues Erastin induced ferroptosis and restores decidualization in telomerase‐immortalized human endometrial stromal cells (T hESCs). (A) Representative transmission electron microscopy (TEM) images of mitochondrial ultrastructure in T hESCs under indicated treatments indicated shrunken mitochondria with condensed membranes, characteristic of ferroptosis. (B–D) Biochemical assessment of ferroptosis markers: Malondialdehyde (MDA) content (B), superoxide dismutase (SOD) activity (C), and the reduced/oxidized glutathione (GSH/GSSG) ratio (D) ( n = 6). (E–H) Representative immunofluorescence images showing intracellular ferrous iron (Fe 2+ ) (E), and expression of TFRC (F), ACSL4 (G), and SLC7A11 (H) (scale bar: 50 μm). (I–L) Quantification of fluorescence intensity for Fe 2+ (I), TFRC (J), ACSL4 (K), and SLC7A11 (L) ( n = 3). (M, N) ELISA was used to quantify the level of IGFBP1 (M) and PRL (N) from each group ( n = 6). Data are presented as means ± SEM. Statistical significance is determined by one‐way ANOVA (Dunnett's post‐test). ** p < 0.01 (normal control vs. model group); # p < 0.05, ## p < 0.01 (treatment groups vs. model group).

Article Snippet: Curcumin (MedChemExpress, Cat. No.: HY‐N0005), Dydrogesterone (MedChemExpress, Cat. No.: HY‐B0257A), Erastin (MedChemExpress, Cat. No.: HY‐15763), JQ‐1 (MedChemExpress, Cat. No.: HY‐13030), 8‐bromoadenosine 3′, 5′‐cyclic monophosphate (8‐Br‐cAMP) (Sigma, Cat. No.: B5386), Medroxyprogesterone acetate (MPA) (APExBIO, Cat. No.: B1510), DMEM/F12 (Thermo Fisher, Cat. No.: 12634010), Fetal bovine serum (FBS) (Gibco, Cat. No.: 10099141); rLV‐CMV‐BRD4‐PGK‐Puro‐WPRE (BrainVTA Co. Ltd., Wuhan, China); CoraLite594‐Phalloidin (red) (Proteintech, Cat. No.: PF00003), FeRhoNox‐1 (Fe 2+ indicator) (Maokangbio, Cat. No.: MX4558); mouse MDA ELISA kit (BYabscience, Cat. No.: BY‐WJZF0071), mouse SOD ELISA kit (BYabscience, Cat. No.: BY‐ EM221637 ), mouse PRL ELISA kit (BYabscience, Cat. No.: BY‐ EM220246 ), mouse IGFBP1 ELISA kit (BYabscience, Cat. No.: BY‐ EM228061 ), human MDA ELISA kit (BYabscience, Cat. No.: BY‐WJZF0118), human SOD ELISA kit (BYabscience, Cat. No.: BY‐ EH112140 ), human PRL ELISA kit (BYabscience, Cat. No.: BY‐ EH110473 ), human IGFBP1 ELISA kit (BYabscience, Cat. No.: BY‐ EH114674 ), GSH ELISA kit (BYabscience, Cat. No.: BY‐WJZF0366), GSSG ELISA kit (BYabscience, Cat. No.: BY‐WJZF1210).

Techniques: Transmission Assay, Electron Microscopy, Activity Assay, Immunofluorescence, Expressing, Fluorescence, Enzyme-linked Immunosorbent Assay, Control